Block of Cleavage of Ultraviolet-irradiated DNA with Restriction Endonucleases

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概要

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• Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restriction endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), and at the position adjacent to the cutting site (Aha III) blocked the cleavage with the enzymes. In addition, experiments with Hind III, Eco RI and Bam HI showed that the extent of cleavage block appears to reflect the relative efficiency of dimer formation in sequences, TT, CT and CC.

• 日本放射線影響学会の論文

著者

• 富田 悟

  東海大学医学部分子生物学教室

• 魏 康

  東海大学医学部分子生物学教室

• 鈴木 ?之

  東海大学医学部分子生物学教室

• 魏 康

  東海大学医学部分子生物学教室:(現)中国予防医学中心工業衛生実験所

関連論文

• Block of Cleavage of Ultraviolet-irradiated DNA with Restriction Endonucleases
• Inhibitory Effect of Irradiation of DNA with Near Ultraviolet Light in the Presence of 8-Methoxypsoralen on Cleavage by Restriction Endonucleases

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