Increase in the Apparent Sensitivity of HeLa Cells on a Membrane Filter to Ultrviolet Radiation

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概要

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• HeLa cells were allowed to become attached to a membrane filter and then irradiated with UV radiation at 254 nm. The viability of the cells was lower than that of cells which were irradiated on a plastic Petri dish. This decrease in viability was observed not only when the cells were in direct contact with the membrane filter, but also when the membrane filter was placed outside the Petri dish containing the HeLa cells which allowed the transmission of UV. Experiments with T4 phage in suspension strongly suggest that this decrease in viability is due to the reflection of 254 nm radiation by the membrane filter. Membrane filters are widely used for the filtration of materials. It has also been demonstrated that cells attached to a membrane filter show no detectable changes in cell morphology or cell growth kinetics1), and cells attached to membrane filters are used as samples for electron microscopy for the analysis of cell morphology1-3).

• 日本放射線影響学会の論文

著者

• 山本 和生

  神戸大学医学部放射線基礎医学教室

• 井上 善夫

  神戸大学医学部放射線医学教室

• 篠原邦 夫

  神戸大学医学部放射線基礎医学教室:東京都臨床医学総合研究所

関連論文

• 肺門リンパ節シンチグラフィー
• Increase in the Apparent Sensitivity of HeLa Cells on a Membrane Filter to Ultrviolet Radiation

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