Presence of an Endonuclease Specific for Ultraviolet Light-irradiated Deoxyribonucleic Acid in a Ultraviolet Light-sensitive Mouse Cell Mutant Q31

スポンサーリンク

概要

• 論文の詳細を見る

• It has been shown that rodent cell lines permanently established in cell culture are unable to excise pyrimidine dimers into acid-soluble form but that they have an activity to nick ultraviolet light (UV)-irradiated DNA in nuclear crude extracts. From mouse cell line L5178Y mutagenized with N-methyl-N'-nitroN-nitrosoguanidine, Sato and Hieda (1979) isolated a clone, designated Q31, which showed a high sensitivity to 4-nitroquinoline-1-oxide and UV but not to X-rays. Sato and Setlow (1981) measured the disappearance of UV-specific endonuclease-sensitive sites from the DNA of UV-irradiated mutant Q31 and parental L5178Y cells and found that Q31 cells were deficient in excision repair of pyrimidine dimers as compared to parental cells. These results indicate that the first incision step of excision repair might be impaired in Q31 cells. Hence we have examined the possibility if the Q31 cells have a defect in an endonuclease specific for UV-irradiated DNA in alkaline sucrose gradients after treatment with nuclear extracts.

• 日本放射線影響学会の論文

著者

• MORIMYO Mitsuoki

  Division of Genetics, National Institute of Radiological Sciences

• Sato Koki

  Division Of Genetics National Institute Of Radiological Sciences

• Morimyo Mitsuoki

  Division Of Chemistry National Institute Of Radiological Sciences

関連論文

• T cell lymphoma cells derived from SCID mouse are sensitive to X-ray Irradiation and beta-rays from ^C In DNA.
• DNA repair mechanisms of S. pombe III. Radiation-resistant gene redM12 encoding a novel ubiquitin-conjugating enzyme E2.
• Cloning of stress inducible genes of S. pombe.
• Presence of an Endonuclease Specific for Ultraviolet Light-irradiated Deoxyribonucleic Acid in a Ultraviolet Light-sensitive Mouse Cell Mutant Q31

スポンサーリンク