Changes of AdenYlate Energy Charge of Hog Blood Lymphocytes, Platelets and Erythrocytes Following Gamma lrradiation

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The effect of gamma irradiation on free nucleotides content of hog blood erythrocytes, platelets and lymphocytes in the dose range of 0.5-500 krad, 1 h after irradiation, was examined. In the dose range of 0.25-7 krad significant changes of AMP level were found in all 3 types of cells. In the dose of 30-500 krad significant changes of adenine nucleotides were observed only for blood platelets, concerning AMP, ADP and ATP. The values of adenylate energy charge indicate acceleration of ATP generating sequences in the dose range of 0.5-5 krad. It is suggested that changes of energy charge belong to the first indices of radiationinduced damage. The purpose of numerous studies was to determine if disturbances in energy metabolism of irradiated cells belong to the first indices of radiation-induced changes. Degradation of adenine nucleotides precedes damage to ATP synthesis. Araki et al. stated the release of hypoxanthine into the extracellular medium due to deamination of adenosine and AMP, already 20 minutes after irradiation of rat thymocytes with doses of 0. 5-32 krad. They stated further that the last step in ATP synthesis i. e. the phosphorylation process is not infiuenced by radiation until one hour following irradiation of rat thymocytes. Yamada and Ohyama suggested, that decrease in ATP content can be partly compensated by increased glycolytic activity below 8 krad of X-irradiation.
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