Characterization of Free DNP Induced by X-irradiation in Rat Spleen

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For the characterization of free DNP (saline-soluble deoxyribonucleoprotein) increasing in rat spleen after wholebody exposure to ionizing radiation, the estimation of the molecular weight of this DNP and the determination of the base composition of the DNA held in this DNA-protein complex were attempted with several analytical methods such as gel filtration, buoyant density centrifugation and paper chromatography. The molecular weight of free DNP as a complex of DNA and protein was in the range of millions. In the present course of experiment, a large contamination of RNA rich in guanine was found in the DNA fraction prepared and purified according to the methods of Swingle and Cole. However, this contaminated RNA resistant to pancreatic RNase could be removed completely by the additional treatment with RNase-T1. The base composition of the DNA held in free DNP or residual DNP. DNP other than free DNP, was not so different from that of DNA prepared from normal spleen of rat. These results were discussed in terms of sub-structure of chromatin.
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