Evidence that a Recombinationless Strain, rad 51, of Saccharomyces cerevisiae Lacks the Budding Cell Resistance to γ-rays
スポンサーリンク
概要
- 論文の詳細を見る
Radiosensitivities to γ-rays of wild-type and an x-ray sensitive mutant, rad 51 (defective in genetic recombination) of Saccharomyces cerevisiae were compared using non-synchronized and partially synchronized cultures. The rad 51 cells, either in haploid or diploid, showed only a very small change in radiosensitivity during the cell growth, whereas wild-type cells, especially in haploid, showed wellknown budding resistance. The heterozygous (wild/rad 51) diploid cells showed a remarkable budding resistance and sigmoidal inactivation kinetics in survival curve similar to wild-type homozygous diploid cells. The budding resistance is a wellknown phenomenon in Saccharomyces cerevisiae regardless of its ploidy. Beam et al first reported an increase in radi-oresistance as the first buds appeared following inoculation of resting cells into fresh growth medium. Studies on synchronous culture of diploid yeast cells showed that the radioresistance appeared soon after the start of the S phase, and maximized at the end of this stage.
- 日本放射線影響学会の論文
著者
関連論文
- Cross-Sensitivity to DNA-Damaging Agents in Radiation-Sensitive Mutants of Murine Leukemia Cells
- Mode of Radiosensitization of Hypoxic Cells by Furylfuramide
- II. Recombinogenesis after the Photoaddition of Furocoumarins : Effects of Post-treatment Incubation on Recombinogenesis in Incision-proficient and Incision-deficient Strains of Saccharomyces cerevisiae:
- I. Recombinogenesis after UV- and γ-Ray-irradiation : Effects of Post-treatment Incubation on Recombinogenesis in Incision-proficient and Incision-deficient Strains of Saccharomyces cerevisiae:
- Evidence that a Recombinationless Strain, rad 51, of Saccharomyces cerevisiae Lacks the Budding Cell Resistance to γ-rays