Activation of Myosin-ATPase by Irradiated Histidine
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概要
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Activation of myosin-ATPase by irradiated histidine was studied. The kinetic constant and the optimum pH for the activated enzyme were found to be different from those of non-activated enzyme. An activating agent was separated from the irradiated histidine solution, through a column chromatography on ion-exchange resin. From its chemical properties and spectral characteristics, this was first assumed to be hydroxyhistidine. However, a peroxide which is more effective in activating the enzyme was also obtained from histidine on irradiation. Oxygen is needed for the production of this peroxide. Based on the studies of its sulfhydryl reactivity and heat stability, it was concluded that the peroxide concerned was not hydrogen peroxide, but presumably hydroxyhydroperoxide of histidine. From comparative studies on the activating effect on myosin of irradiated histidine and other sulfhydryl reagents, it was confirmed that irradiated histidine attacks a sulfhydryl group which is concerned with the conformation of myosin molecules, leading to an activation of myosin-ATPase.
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