β_2-インデグランドに会合する新規ヒトGPIアンカー蛋白質(GPI-80)に対する単クローン抗体はヒト単球の macropinocytosis を選択的に増強する

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We have shown previously that neutrophils and small population of human monocytes have a glycosylphosphatidylinositol (GPI) -anchored protein, GPI-80 on their surface. In a study of neutrophils, we reported that GPI-80 regulates the adherence and migration. In this study, we examined the functions of GPI-80 in monocytes. Treatment of monocytes with monoclonal antibody (mAb) to GPI-80 enhanced pinocytosis of fluorescein isothiocyanate (FITC)-labeled dextran but not that of lucifer yellow and low-density lipoprotein. This treatment neither changed phagocytosis, adherence nor reactive oxygen production of monocytes. These results suggest that GPI-80 modulates fluid phase macropinocytosis of high molecular weight molecules, but not that of low molecular weight ones, clathrin-dependent pinocytosis, phagocytosis, adherence and reactive oxygen production. Treatment of neutrophils with mAbs to GPI-80 did not change macropinocytosis of FITC-labeled dextran, suggesting that the functions of GPI-80 on macropinocytosis of high molecular weight molecules are different between neutrophils and monocytes. This study, first reported GPI-80 function on monocytes, implies that GPI-80 may be one of the molecules associated with macropinocytosis on monocytes.　Key words: macropinocytosis, monocytes, GPI -anchored protein
山形大学の論文
2005-02-16