25 トマトの単為結実性果実のアポプラスト中のIAA、GA_1及びCKs
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概要
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Levels of indole-acetic acid (IAA), cytokinins (CKs) and gibberellic acid1 (GA_1) in seedless fruits of parthenocarpic tomato (cv. Rarkuna First) were analyzed. CKs included trans-zeatin (Z) and trans-zeatin riboside (ZR). Fruits were sampled at young stage (6 cm in a diameter) and mature green stage (8 cm in a diameter). Sampled fruits were sliced from equatorial region in 20% thickness of total fruit thickness, and sliced disks were separated into parts. The pericarps and partitions tissues were centrifuged for collection of apoplast solution (AP; sap out of a cell membrane). Thereafter the tissues were frozen, thawed and recentrifuged for collection of symplast solution (SP; sap within a cell). The locule tissues were homogenated and filtrated. At extraction, ^<13>C_6-IAA, ^2H_5-Z, ^2H_5-ZR, ^2H_2-GA_1 as internal standards were added. After purification, IAA, CKs and GA_1 were fractionated with HPLC and determined with LC-MS. IAA concentration was generally higher in 6cm-fruit than in 8cm-fruit. CKs concentrations were higher in partitions and locules tissues of 6cm-fruit (especially Z was 4.6 pmol/g fresh weight in SP of partition tissues and ZR was 55 in locule tissues), suggesting that partitions of fruits may be the site of Z synthesis. The concentrations of plant hormones in divided parts changed differently. Thus, precise determination of the hormone concentrations of the component tissues needs to assign specific roles in specific tissues.
- 植物化学調節学会の論文
- 2002-10-31
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関連論文
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- トマトの単為結実性果実のアポブラスト中のIAA,GA_1及びCKs(第37回大会研究発表抄録)
- 25 トマトの単為結実性果実のアポプラスト中のIAA、GA_1及びCKs
- 単為結実性トマト果実の内生植物ホルモン
- 植物ホルモンの定量のためのLC-MS-SIMの設定
- 46.植物ホルモンの分析のためのLC-MS-SIMの高感度の設定条件(第36回大会研究発表抄録)