82 糖代謝におけるNPK5プロテインキナーゼの役割

概要

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Tobacco NPK5 is a functional homologue of yeast SNF1 protein kinase, which plays a central role in catabolite repression in yeast cells. To elucidate the function of NPK5 on the regulation of sugar metabolism in plants, transgenic tobacco plants that expressed sense NPK5, antisense NPK5 and kinase-negative NPK5 were produced. Isolated roots of kinase-negative NPK5 transformants were rapidly elongated depend on sucrose concentration. The expression of sucrose synthase was repressed in the transformants. On the other hand, PRL1 gene results in glucose hypersensitivity in Arabidopsis. AKIN10, a homologue of tobacco NPK5 was found to interact with PRL1 in the yeast two-hybrid system. Kinase activity of AKIN10 was analyzed in both wild and prl1 mutant Arabidopsis. In comperation with wild type, the kinase activity was higher in the prl1 mutant, suggesting that PRL1 is a negative regulater of the kinase.
1999-10-01