27 エチレン応答性転写因子タバコERF3と相互作用するタンパク質の単離とその解析

概要

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Plants produce a number of pathogenesis-related (PR) proteins in response to pathogen invasion. Induction of one group of PR genes is mediated by plant hormone ethylene through a consensus cis-sequence in the promoter region known as GCC-box. Tabacco ERFs (EREBPs; ethyleneresponsive element binding proteins) have been identified as GCC-box binging proteins and may have crucial roles in the regulation of ethylene-induced PR gene expression. To elucidate the post-translational regulation of ERFs involved in the ethylene-responsive expresson of PR genes, we used ERF3 as a bait in the yeast two-hybrid system. A clone b8 was isolated as a physical interacting protein with ERF3 from cDNA library prepared from cultured tobacco cells. Interestingly, the DNA binding domain of ERF3 was not necessary for the interaction with the clone b8 in yeast. These results suggest that the novel mode of post-translational modification of ERF3 may be involved in the GCC-box-mediated PR gene expression. The gene expression of the clone b8 was detected in all tissues examined; upper leaves, lower leaves, stems, roots, seedlings and cultured cells. Further analysis to elucidate the role of the clone b8 in the ethylene-responsive PR gene expression are on going.
植物化学調節学会の論文
1999-10-01