Functions of PRK1, a Receptor-Like Kinase of Petunia inflata : Phenotypes of the Transgenic Plants Expressing the Antisense PRK1 Gene(Physiology of Pollination and Fertilization, For Further Development of Horticulture in East Asia)

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概要

• 論文の詳細を見る

• We previously identified and characterized a predominantly pollen-expressed gene of Petunia inflata that encodes a receptor-like kinase named PRK1. The extracellular domain of PRK1 contained leucine-rich repeats, and the cytoplasmic kinase domain was found to autophosphorylate on serine and tyrosine. To investigate the function of PRK1 in pollen development, we transformed P. inflata plants with a construct containing the promoter of a predominantly pollen-expressed gene of tomato, LAT52, fused to an antisense PRK1 cDNA. Three transgenic plants produced approximately equal amounts of normal and aborted pollen. Progeny analysis revealed that the transgene cosegregated with the pollen abortion phenotype. The mutant microspores of the transgenic plants developed normally until the uninucleate stage, but were not able to carry out microspore mitosis and eventually disintegrated. Northern blot analysis suggested that the pollen abortion phenotype most likely resulted from down-regulation of the PRK1 gene by the antisense PRK1 transgene. Progeny analysis in two of the transgenic plants, ASRK-13 and ASRK-20, showed that the transmission of the antisense PRK1 gene was also greatly reduced through the female. Light microscopic examination revealed that embryo sac development of approximately half of the ovules of ASRK-13 was abnormal. The majority of the affected ovules were able to progress through most of megagametogenesis to produce a seven-cell embryo sac, but they failed to complete the maturation stages of the embryo sac development when cell expansion, nuclear migration, and differentiation take place. These ovules could not achieve fertilization and subsequently degenerated, resulting in abortion of half of the seeds. RNA gel blot results showed that the PRK1 gene is expressed in the ovary. These results suggest that PRK1 plays an essential role in a signal transduction pathway that mediates post-meiotic development of microspores as well as embryo sac.

• 園芸学会の論文
• 1998-11-15

著者

• Kao Teh-hui

  Department Of Biochemistry And Molecular Biology Pennsylvania State University

• Kao Teh

  Department Of Biochemistry And Molecular Biology The Pennsylvania State University

• Pai Hyun

  Korea Research Institute of Bioscience and Biotechnology

• Mariani Celestina

  Department of Experimental Botany, Catholic University of Nijmegen

• Mariani Celestina

  Department Of Experimental Botany Catholic University Of Nijmegen

• Pai Hyun-sook

  Korea Research Institute of Bioscience and Biotechnology

関連論文

• ウルグアイ以外の地域におけるPetunia axillaris(ナス科)の自家(不)和合性の分布
• Differentiation in the status of self-incompatibility among Calibrachoa species (Solanaceae)
• Functions of PRK1, a Receptor-Like Kinase of Petunia inflata : Phenotypes of the Transgenic Plants Expressing the Antisense PRK1 Gene(Physiology of Pollination and Fertilization, For Further Development of Horticulture in East Asia)
• ペチュニア属(ナス科)分類群における自家不和合性の分化
• Isolation and Expression Analysis of a Tobacco AINTEGUMENTA Ortholog (NtANTL)

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