FLOW CYTOMETRIC ANALYSIS ON LINEAGE-SPECIFIC CELL SURFACE ANTIGENS OF RAT BONE MARROW TO SEEK POTENTIAL MYELOTOXIC BIOMARKERS : STATUS AFTER REPEATED DOSE OF 5-FLUOROURACIL
スポンサーリンク
概要
- 論文の詳細を見る
Flow cytometry (FCM) analysis of CD45, CD45R, CD71 and CD90 expression on Crj : CD (SD) IGS rat bone marrow cells was done after 5-fluorouracil (5-FU) administration to examine whether these lineage-specific cell surface antigens could be myelotoxic biomarkers. The expression of CD45 (CD45^<Low> and CD45^<High> : differing in expression intensity), CD45R, CD71 and CD90 on bone marrow cells coincided with previous reports. After repeated administration of 5-FU at 50 mg/kg/day for 1-5 days, a time-dependent decrease in cells expressing CD45^<Low>, CD71 and CD90 was observed, whereas a decrease in the CD45^<High> expressing cells was not observed. Furthermore, the decrease was dose-dependent in CD45^<Low>, CD71 and CD90 expressing cells after administration of 5-FU between 2 and 50 mg/kg/day for 4 days. After 4-day repeated dose of 5-FU at 50 mg/kg/day followed by a recovery period, the change in number of CD45^<Low>, CD45R, CD71 and CD90 cells to the bottom and in recovery showed different kinetics. In contrast, the change in number of CD45^<High> cells was minimal, and relatively stable after 5-FU administration. The results suggest that CD45, CD45R and CD90 could each be potential myelotoxic biomarkers for a total proportion of common leukocytes including T- and B-lymphocytes, for a total proportion of B-lymphocytes, and for a total proportion of T-lymphocytes plus immature B-lymphocytes and common progenitor cells, respectively. CD71 could be a single myelotoxic biomarker for erythroid cells. Further study is required for isolation of each of the myelo-lymphocytic lineages. However, the present study showed that FCM analysis could be available to assess the lineage or differentiation stage-specific response, such as the different extent and time-course or the kinetics (the time to reach the bottom and to recover to the normal level) of myelotoxic effect in rat bone marrow.
- 日本トキシコロジー学会の論文
- 2004-05-28
著者
-
Ogata Shoko
Pharmacology And Safety Research Department Pharmaceutical Development Research Laboratories Teijin
-
KAKIUCHI Satoko
Pharmacology and Safety Research Department, Pharmaceutical Development Research Laboratories, Teiji
-
OHARA Sachiko
Pharmacology and Safety Research Department, Pharmaceutical Development Research Laboratories, Teiji
-
MIURA Daishiro
Pharmacology and Safety Research Department, Pharmaceutical Development Research Laboratories, Teiji
-
KASAHARA Yoshinori
Pharmacology and Safety Research Department, Pharmaceutical Development Research Laboratories, Teiji
-
IZAWA Yoshihiro
Pharmacology and Safety Research Department, Pharmaceutical Development Research Laboratories, Teiji
-
Ohara Sachiko
Pharmacology And Safety Research Department Pharmaceutical Development Research Laboratories Teijin
-
Izawa Yoshihiro
Pharmacology And Safety Research Department Pharmaceutical Development Research Laboratories Teijin
-
Kakiuchi Satoko
Pharmacology And Safety Research Department Pharmaceutical Development Research Laboratories Teijin
-
Kasahara Yoshinori
Pharmacology & Safety Research Department Pharmaceutical Development Research Laboratories Teiji
-
Kasahara Yoshinori
Pharmacology & Safety Research Department Pharmaceutical Development Research Laboratories Teiji
-
Kasahara Yoshinori
Pharmaceutical Development Research Lab. Teijin Pharma Limited
-
Ogata Syoko
Pharmacology And Safety Research Department Pharmaceutical Development Research Laboratories Teijin
-
Miura Daishiro
Teijin Limited
-
Miura Daishiro
Pharmacology and Safety Research Department, Pharmaceutical Development Research Laboratories TEIJIN Pharma Limited
関連論文
- Examination of immunosuppression by rat peripheral lymphocytes phenotyping : basic examination using rat peripheral blood after dosing DMBA, benzene, EGME (IMMUNE AND ALLERGIC SYSTEM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annua
- P12-04 Basic examination a reticulocytes count using a flow-cytometer (EPICS XL-MCL) : A study of quality control method using Retic-Chex as a control material.
- P-78 Basic Study on the Using Flow-cytometer (EPICSXL) for the Bone Marrow Examination.(Proceedings of the 27th Annual Meeting)
- 21B-10-3 Hematological and blood chemical study of the inhibitory effect on the hematopoietic system in the chemotherapeutics administered to rabbit.
- 21B-09-3 Basic Examination of Reticulocytes Count Using the Flow-cytometer (EPICS XL) : A Comparative Study Between Flow-cytometer Method and Conventional Method Using Hemolytic-anemia Animals.
- FLOW CYTOMETRIC ANALYSIS ON LINEAGE-SPECIFIC CELL SURFACE ANTIGENS OF RAT BONE MARROW TO SEEK POTENTIAL MYELOTOXIC BIOMARKERS : STATUS AFTER REPEATED DOSE OF 5-FLUOROURACIL
- Data characteristic of in vitro micronucleus test in CHL cells(Mutagenicity, Proceedings of the 32nd Annual Meeting)
- DNA MICROARRAY ANALYSIS OF PULMONARY FIBROSIS THREE MONTHS AFTER EXPOSURE TO PARAQUAT IN RATS
- FLOW CYTOMETRIC ANALYSIS OF ERYTHROPOIETIC ABNORMALITY : CHANGES IN THE CELL MATURITY INDEX OF RETICULOCYTES AND RETIC DISTRIBUTION INDEX ARE USEFUL AS INDICATORS OF ERYTHROPOIETIC TOXICITY IN NON-CLINICAL STUDIES
- NOVEL AND EXTENSIVE ASPECTS OF PARAQUAT-INDUCED PULMONARY FIBROGENESIS : COMPARATIVE AND TIME-COURSE MICROARRAY ANALYSES IN FIBROGENIC AND NON-FIBROGENIC RATS
- GENE EXPRESSION ANALYSIS OF THE LUNG FOLLOWING PARAQUAT ADMINISTRATION IN RATS USING DNA MICROARRAY
- Chromosomal counting and DNA-content determination in CHL cells exposed by Vincristine or K252a known as inducers of numerical chromosome aberration. (MUTAGENICITY) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)