Abnormal Embryo Development and Efficient Embryo Rescue in Interspecific Hybrids, Oryza sativa × O. minuta and O. sativa × O. officinalis
スポンサーリンク
概要
- 論文の詳細を見る
Interspecific hybrids between cultivated rice (Oryza sativa, 2n = 24, AA) and two wild species (O. minuta, 2n = 48, BBCC and O. officinalls, 2n = 24, CC) have been produced by cross pollination. The embryo devel-opment in these interspecific hybrids was observed to study the deterioration features in detail. All hybrids showed either abnormal embryo development with degenerating endosperm or embryo retardation itself. Abnormally developed embryos were observed by the end of 7 days after pollination and the percentage of abnormally developed embryos increased from 7 to 14 DAP. The embryos were excised at 7 to 14 DAP and cultured on MS medium supplemented with 1,3 and 5 mg/l benzyl-aminopurine, 1 g/l casein hydrolysate, 0.8% agar and 3% sucrose. The optimal time for rescue turned out to be 11-14 DAP. There was a significant difference (P = 0.05) in the regeneration efficiency rate with rescue time but no significant difference with of culture medium in these cross combinations. The F_1 hybrids were morphologically intermediate between their parents. All F_1 hybrid plants thus obtained were completely sterile. Chromosome doubling was attempted by treating F_1 hybrid tillers with colchicine to overcome their sterility. The morphology of the colchicine-treated F_1 plants closely resembled the female parent and fertility.
- 日本育種学会の論文
著者
-
Adachi Taiji
Laboratory Of Plant Genes And Physiology Division Of Agricultural And Environmental Sciences Graduat
-
Suputtitada Saowanee
Department of Genetics, Faculty of Science, Kasetsart University
-
Pongtongkam Pradit
Department of Genetics, Faculty of Science, Kasetsart University
-
Rodrangboon Pannanee
Laboratory of Plant Breeding, Division of Applied Genetics and Biotechnology, Faculty of Agriculture
-
Rodrangboon Pannanee
Laboratory Of Plant Breeding Division Of Applied Genetics And Biotechnology Faculty Of Agriculture M
-
Suputtitada Saowanee
Department Of Genetics Faculty Of Science Kasetsart University
-
Pongtongkam Pradit
Department Of Genetics Faculty Of Science Kasetsart University
関連論文
- Breeding Barriers in the Interspecific Cross of Oryza sativa L.and Oryza minuta Presl.
- Ultrastructural Aspects on Degeneration of Embryo, Endosperm and Suspensor Cells following Interspecific Crosses in the Genus Fagopyrum
- Abnormal Embryo Development and Efficient Embryo Rescue in Interspecific Hybrids, Oryza sativa × O. minuta and O. sativa × O. officinalis
- Failure of Fertilization Associated with Absence of Zygote Development in the Interspecific Cross of Fagopyrum tataricum × F. esculentum
- Inheritance and Characterization of a Null Allele for Group A Acetyl Saponins found in a Mutant Soybean (Glycine max (L.) Merrill)
- Conversion of AFLP Markers Linked to the S^h Allele at the S Locus in Buckwheat to a Simple PCR Based Marker Form