Detection of Alien Genes and Analysis of their Integration Position in Transgenic Rice by Fluorescence in situ Hybridization

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概要

• 論文の詳細を見る

• Using fluorescence in situ hybridization(FISH), we localized transferred barnase-ps1 and pHctinG DNA sequences onto chromosomes in a group of rice transgenic plants cotransformed by microprojectile bombardment. In all of the tested rice transgenic lines, the detected cells showed 1-3 signal spots and they were located in the central and terminal regions of chromosome arms. The signals of both barnase-ps1 and pHctinG genes were mostly detected in close contact on chromosomes, which could not be resolved without using two-color in situ hybridization. In a few cells, the signals were separated from each other for barnase-ps1 and pHctinG.

• 日本育種学会の論文

著者

• Ling Ding-hou

  South China Institute Of Botany The Chinese Academy Of Sciences

• Song Yun-chun

  Key Lab Of Moe For Plant Developmental Biology Wuhan University

• Jin Wei-wei

  Key Lab Of Moe For Plant Developmental Biology Wuhan University

• Ning Shun-Bin

  Key Lab of MOE for plant Developmental Biology

• Qin Rui

  Key Lab of MOE for plant Developmental Biology

• Huang Si-Luo

  Key Lab of MOE for plant Developmental Biology

• Qin Rui

  Key Lab Of Moe For Plant Developmental Biology Wuhan University

• Huang Si-luo

  Key Lab Of Moe For Plant Developmental Biology Wuhan University

• Ning Shun-bin

  Key Lab Of Moe For Plant Developmental Biology Wuhan University

関連論文

• 形質転換イネにおける外来遺伝子の蛍光in situハイブリダイゼーションによる挿入位置の解析
• Detection of Alien Genes and Analysis of their Integration Position in Transgenic Rice by Fluorescence in situ Hybridization

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