Benzyladenine-preconditioning in Germinating Mungbean Seedlings Stimulates Axillary Buds in Cotyledonary Nodes Resulting in Multiple Shoot Regeneration
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概要
- 論文の詳細を見る
Tissue culture and histological studies were done to ascertain the effects of benzyladenine(BA)-preconditioning on direct shoot regeneration from cotyledonary node explants of mungbean. The highest direct shoot regeneration(93.3%), double-sided shoot initiation(65.0%)and average number of shoots per explant(2.7 shoots)were obtained when Pag-asa 7 explants are excised from seedlings germinated in basal medium(BM)consisting of MS salts and B5 vitamins with 1.0 mgl^<-1> BA, and subsequently cultured in fresh medium of the same composition. Histological inspection of sections from germinating seeds in BM and BM + 1.0 mgl^<-1> BA sampled at 1 to 4 days from germination revealed bigger and developmentally more advanced axillary shoots in BA-preconditioned seedlings, a built-in advantage which could attest to the improved regenerability in BA-treated seddling explants over the control(BM only). During shoot initiation from explants, two treatments namely doubling of the concentration BA to 2.0 mgl^<-1> and the addition of a non-ionic surfactant, Pluronic F-68 did not further increase the responses, but gave results comparable with those obtained using BM + 1.0 mgl^<-1> BA alone. Conversely, a BA-thidiazuron(TDZ)combination(each at 1.0 mgl^<-1>)when added to BM significantly reduced shoot induction to only 48.3% possibly due to increased callusing at the nodes in 55% of the explants. The importance and applicability of the regeneration system in tissue culture and/or genetic manipulations of mungbean and other Asiatic Vigna species is discussed.
- 日本育種学会の論文
著者
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Hattori Kazumi
Laboratory Of Plant Genetics And Breeding Graduate School Of Bioagricultural Sciences Nagoya Univers
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Avenido Renato
Laboratory of Plant Genetics and Breeding, Graduate School of Bioagricultural Sciences, Nagoya Unive
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Hattori Kazumi
Laboratory Of Plant Genetics And Breeding Graduate School Of Bioagricultural Sciences Nagoya Univers
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Avenido Renato
Laboratory Of Plant Genetics And Breeding Graduate School Of Bioagricultural Sciences Nagoya Univers
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