ADVANTAGES OF IN VITRO CYTOTOXICITY TESTING BY USING PRIMARY RAT HEPATOCYTES IN COMPARISON WITH ESTABLISHED CELL LINES
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概要
- 論文の詳細を見る
We investigated and compared the cytotoxicity of 16 reference compounds in four in vitro systems: primary cultured rat hepatocytes, hepatoma HepG2 cell line, non-hepatic HeLa and Balb/c 3T3 cell lines. After 24 hr of exposure to the test compounds, the water-soluble tetrazolium salts WST-l assay was used as an endpoint to evaluate cytotoxicity. Acetaminophen, diclofenac sodium cyclophosphamide and disulfiram displayed from 2 to more than 10 times higher IC_<50> values in three cell lines than in rat primary cultured hepatocytes. The cytotoxic effects of aspirin, amiodarone, clorfibiric acid, chlorpromazine, erythomycin, lithocholic acid, cisplatin and quinidine in rat hepatocytes were similar or 2 times stronger than those observed in cell lines. Ketoconazole resulted in the lowest IC_<50> value in the HeLa cell line. The data suggested that the compounds which are known to be metabolism-mediated liver toxicants have a differential hepatotoxicity in vitro and that primary cultured rat hepatocytes could represent a valuable tool for both screening and study of the effects of bio-transformation on the cytotoxicity of new chemical entities and xenobiotics in vitro.
- 日本トキシコロジー学会の論文
- 2002-08-22
著者
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Wang Kun
Department of Pediatric Surgery, Juntendo University School of Medicine
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Shindoh Hidetoshi
中外製薬
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Shindoh Hidetoshi
Department Of Preclinical Science Nippon Roche Research Center
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Inoue Tomoaki
Department of Toxicology and Pathology, Nippon Roche Research Center
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Wang Kun
Department Of Pediatric Surgery Juntendo University School Of Medicine
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Wang Kun
Department Of Pharmacology Dalian Medical University
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Inoue Tomoaki
Department Of Preclinical Science Nippon Roche Research Center
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HORII lkuo
Department of Preclinical Science, Nippon Roche Research Center
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Horii Lkuo
Department Of Preclinical Science Nippon Roche Research Center
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Inoue Tomoaki
Department Of Applied Molecular Chemistry College Of Industrial Technology Nihon University
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