COLLABORATIVE STUDY ON RAT SPERM MOTION ANALYSIS USING CELLSOFT SERIES 4000 SEMEN ANALYZER
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概要
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A collaborative study was conducted to determine useful and sensitive rat sperm motion parameters in a CellSoft Series 4000 semen analyzer to detect the effects of compounds on sperm motion. The effects on the sperm motion parameters were investigated using α-chlorohydrin, boric acid, ethinylestradiol, ethyl methanesulfonate, nitrazepam, nitrobenzene, ornidazole, sulfasalazine or valproic acis which are well known to induce reproductive or testicular toxicities. All compounds used in this study decreased percentage of motile sperm (% motile). Curvilinear velocity (VCL), maximum and mean amplitude of lateral head displacement (ALH max and ALH mean) were decreased by treatment with all compounds except for valproic acid. Treatment with α-chlorohydrin, ornidazole or sulfasalazine under mid-dosage regimens decreased only these parameters. Beat cross frequency (BCF) was increased by treatment with sulfasalazine. There were some treatments which caused either decreased or increased changes irrespective of dosage regimen in linearity, average radius, percentage of circular-swimming sperm out of motile sperm (circular/motile) and percentage of circular-swimming sperm out of all sperm (circular/all). Based on these results, we concluded that % motile, VCL, ALH max and ALH mean are considered useful and sensitive parameters for evaluating the effects of compounds on sperm motion. A parameter of BCF can be useful to detect the effects of specific compounds on sperm motion. Linearity, average radius, circular/motile and circular/all are not considered useful or sensitive indicators to detect the effects of compounds on sperm motion.
- 2001-02-20
著者
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BAN Yoshiki
Safety Assessment, Development and Research Laboratories, Banyu Pharmaceutical Co., Ltd.
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NAYA Masato
Toxicological Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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NISHIMURA Tatsuya
Fukui Institute for Safety Research, Ono Pharmaceutical Co., Ltd.
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KANETO Masako
Developmental Research Laboratories, Shionogi & Co., Ltd.
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KISHI Kurajiro
Developmental Research Laboratories, Shionogi & Co., Ltd.
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INOUE Tadahiro
Pharmaceutical Research Laboratories, Taisho Pharmaceutical Co., Ltd.
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YOSHIZAKI Hiroshi
Drug Safety Research Labs, Hikari Branch, Takeda Chemical Industries, Ltd.
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OOSHIMA Yojiro
Drug Safety Research Labs, Takeda Chemical Industries, Ltd.
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Kishi Kurajiro
Developmental Research Laboratories Shionogi & Co. Ltd.
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Ban Yoshiki
Safety Assessment Banyu Pharmaceutical Co. Ltd.
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Kaneto Masako
Developmental Research Laboratories Shionogi & Co. Ltd.
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Inoue Tadahiro
Pharmaceutical Research Laboratories Taisho Pharmaceutical Co. Ltd.
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Inoue Tadahiro
Pharmaceutical Research Laboratories Taisho Pharmaceutical Co. Ltd
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Naya Masato
Toxicological Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Naya Masato
Toxicological Research Laboratories Kyowa Hakko Kogyo Co. Ltd Ube
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Ooshima Yojiro
Drug Safety Research Labs Takeda Chemical Industries Ltd.
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Yoshizaki Hiroshi
Drug Safety Research Labs Hikari Branch Takeda Chemical Industries Ltd.
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Nishimura Tatsuya
Fukui Institute For Safety Research Ono Pharmaceutical Co. Ltd.
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Ooshima Yojiro
Drug Safety Research Center Pharmaceutical Research Division Takeda Chemical Industries
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