THE EFFECT OF FOOD CHEMICALS ON CELL AGING OF HUMAN DIPLOID CELLS IN IN VlTRO CULTURE
スポンサーリンク
概要
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The potency of food chemicals to induce cell aging was evaluated in human diploid fibroblast cells HAIN-55 having a finite replicative potential by using in vitro aging markers, i.e., decreases of maximum proliferative potential (lifespan) of cells, saturation density in monolayer culture (SD), plating efficiency (PE) and mitotic index (MI), and an increase of cells with polyploid karyotypes. By treatment twice with low concentration of genotoxic chemicals aflatoxin B_1, allylisothiocyanate or trans-cinnamaldehyde (severe clastogenic flavoring agent; Kasamaki et al., 1982), lifespan (expressed by the number of cumulative cell population doubling (CPD)) of the treated cells was reduced by 8-12 CPDs accompanied by change of the other aging markers. By successive treatment (29 or 25 times) with non-genotoxic chemical aspartame (N-L-aspartyl-L-phenylalanine) or L-canavanine (structural analogue of L-arginine), lifespan of the treated cells was also slightly shortened (by 2-6 CPDs) compared with the untreated control cells. In the process of cell aging, Mitochondrial activity (MTT activity) decreased almost in parallel with the decrease of SD and MI. On the basis of these results, a variety of genotoxic and non-genotoxic chemicals were examined by using MTT activity as the aging marker for their effects on the aging of HAIN-55 cells and bovine artery endotherial cells which also had a finite replicative potential. The results showed that seven genotoxic and nine non-genotoxic chemicals promoted cell aging.
- 日本トキシコロジー学会の論文
- 1993-08-25
著者
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Kasamaki Akiko
Department Of Hygiene Sapporo Medical College
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Kasamaki Akiko
Department Of Hygiene And Epidemiology Sapporo Medical College
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Urasawa Shozo
Department Of Hygiene Sapporo Medical University School Of Medicine
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Urasawa Shozo
Department Of Hygiene Sapporo Medical College
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Urasawa Shozo
Department Of Hygiene And Epidemiology Sapporo Medical College
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- THE EFFECT OF FOOD CHEMICALS ON CELL AGING OF HUMAN DIPLOID CELLS IN IN VlTRO CULTURE
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