STUDIES ON EXPERIMENTAL CONDITIONS FOR DETECTING PHOTOTOXIC POTENTIALS OF DRUGS IN Balb/c MICE

概要

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This study was designed to establish a procedure for detecting the phototoxicity of drugs in an animal model. Experimental conditions in relation to intensity distribution of ultraviolet-A (UVA), duration of irradiation, and suitable region for irradiation were investigated. One black light gave a wide constant-energy region when the distance from the light source to the irradiation area was 15 cm. The intensity distribution of a bank of 10 black lights formed a pattern like the contour map of a truncated cone in the irradiation area. In phototoxic studies, Balb/c strain mice were orally administered chlorpromazine and nalidixic acid, clinically known as photosensitizers, and were immediately exposed to UVA irradiation. The optimal irradiation time was 4 hours at an energy of 20 Joules/cm^2, which with a high frequency caused erythema on the surface of the ears in the central area, which received about 1.5 mW/sec・cm^2, but no reaction occurred in the surrounding area (0.5-0.8 mW/sec・cm^2). These results indicate that it is important to select a suitable irradiation area and sufficient intensity of irradiation in order to determine whether a drug has phototoxic potential.
日本トキシコロジー学会の論文
1989-08-25