Efficient Production of Transgenic Soybean using a Co-transformation Method

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概要

• 論文の詳細を見る

• Embryos of Glycine max (L.) Merrill cv. 'Jack' were cotransformed with the hygromycin phosphotransferase (hpt) gene and the jellyfish green fluorescent protein (GFP) gene by microprojectile bombardment. The purpose of this study was to determine the optimum conditions which could achieve the highest co-transformation rate using this method. In the whole process of cotransformation, the screening of transformants was effectively monitored by the expression of GFP. Transformation rates were highest when (1) induced embryos were transferred to a solid medium more than 5 times, (2) only the most highly-proliferated embryos forming globular clusters were bombarded, (3) the helium pressure of rupture disc was 1,100 psi, and (4) the embryogenie tissues 5 days after bombardment were cultured for more than 8 weeks in hygromycin-containing liquid medium for selection. In these conditions, the proportion of clones expressing hygromycin-resistance was 7 %, and the proportion of GFP-expressing clones was 18% of them.

• 日本育種学会の論文

著者

• Hidaka Soh

  National Agricultural Research Center For Tohoku Region

• Furutani Noriyuki

  Kyoto Prefectural Institute of Agricultural Biotechnology

関連論文

• Structural Variations of the Aldolase Gene Region in Different Rice Cultivars
• Cell Type- and Positionally Specific Regulation of the Aldolase P Gene Expression in Rice Seedlings
• Soybean Mosaic Virus Resistance in Transgenic Soybean Caused by Post-transcriptional Gene Silencing
• Coat Protein Gene-Mediated Resistance to Soybean Mosaic Virus in Transgenic Soybean
• Efficient Production of Transgenic Soybean using a Co-transformation Method

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