Isolation of Carp Complement Factor B and Formation of the C3-Convertase
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概要
- 論文の詳細を見る
Complement factor B (Bf) is a keycomponent that forms a catalytic side of C3-convertase of the alternative activation pathway. However, the functional assay and isolation method has been developed poorly for Bf in fish. We describe here a hemolytic assay method for Bf of carp (Cyprinus carpio). The assay derised from prified carp C3 and factor D (Df) is based on the action of Bf which cleaves C3 in the presence of Df. Using this assay method as a probe, carp Bf was isolated from serum, and proved to be a protein comopsed of a single polypeptide with a molecular mass of 93 kDa. This C3-convertase of tje alternative pathway of carp completement was assembled from purified C3, Bf and generation of C3b, the initial biologically active fragment derived from C3, was confirmed, suggesting a well conserved mechanism of the formation of the alternative pathway C3-convertase.
- 九州大学の論文
著者
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Nakao Miki
Laboratory Of Marine Biochemistry Department Of Bioscience And Biotechnology Kyushu University
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Nakao Miki
Laboratory Of Marine Biochemistry Division Of Marine Biological Chemistry Department Of Bioscience A
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Nakao Miki
Laboratory Of Fisheries Chemistry Faculty Of Agriculture Kyushu University
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Yano Tomoki
Laboratory Of Marine Biochemistry Department Of Bioscience And Biotechnology Kyushu University
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Yano Tomoki
Laboratory Of Marine Biochemistry Division Of Marine Biological Chemistry Department Of Bioscience A
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Yano Tomoki
Laboratory Of Fisheries Chemistry Faculty Of Agriculture Kyushu University
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Nakahara Makiko
Laboratory of Marine Biochemistry,Division of Marine Biological Chemistry,Department of Bioscience a
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Nakahara Makiko
Laboratory Of Marine Biochemistry Division Of Marine Biological Chemistry Department Of Bioscience A
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