MTT assay を用いた抗癌剤感受性試験のヒト肺癌細胞に対する検討

概要

論文の詳細を見る
A simple colorimetic test, the MTT assay, has been adapted for chemosensitivity testing of a human lung adenocarcinoma cell line, A549, and 25 lung cancer specimens clinically obtained by radical surgery. Seven different chemotherapeutic agents [cisplatin (CDDP) , carboplatin ( CBDCA), mitomycin C (MMC), adriamycin (ADM), etoposide (VP-16), vindesine (VDS) , and 7-ethyl-10-hydroxyl camptothecin (SN-38)] were tested. Minced tumor tissues were treated with collagenase/DNase in RPMI 1640 medium containing 10% fetal calf serum for 1 h at 37℃. These cells were plated in 96-well plates at 1.0 × 10^4 cells per well. The MTT assay was carried out following incubation of the cells with each antitumor agent for 72 h in humidified 5% CO 2 atmosphere at 37℃. The drug concentrations were determined on the basis of theoretical peak plasma concentrations (PPC) proposed by Scheithauer et al. The growth inhibition rates and IC_<50> (the concentration of drug that caused 50% reduction in absorbance compared to baseline values) were used for evaluation of the chemosensitivity. The growth inhibition rates of A549 and lung cancer specimens to all drugs except VDS gradually increased in a drug concentration-dependent manner as well as in an exposure time-dependent manner. The mean IC_<50> values of 25 lung cancer specimens were : MMC : 1.40±1.77 PPC, SN-38 : 2.15±3.19 PPC, ADM : 2.49±2.76 PPC, VP-16 : 3. 17±1.15 PPC, CDDP : 5.26±3.29 PPC, and CBDCA : 5.35±2.27 PPC. Although IC_<50> Values of clinical samples were highly variable, MMC and SN-38 showed a strong antitumor activity. Thus it was concluded that MMC and SN-38 might be useful chemotherapeutic agents for human lung cancer.
日本医療薬学会の論文
1996-08-10

MTT assay を用いた抗癌剤感受性試験のヒト肺癌細胞に対する検討

スポンサーリンク

概要

著者

関連論文

スポンサーリンク