Subgroup-Specific Detection of Cucumber Mosaic Virus RNA in Tobacco Leaves by Tissue-Printing

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概要

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• Experimental conditions for tissue-printing of Cucumber mosaic virus （CMV） RNA were determined to analyze kinetics of viral multiplication in tobacco leaves. Tissue-printing technique usually needs a step of peeling epidermis of the leaves. However, the step is an obstacle to obtain reliable results. In this study, treatments with enzyme and freeze-thaw were used as alternatives of the peeling step. The results obtained here suggest that enzyme treatment is an appropriate substitution for the peeling of epidermis and that the optimum incubation time for enzyme reaction is for 4－6 hours. We found no cross-reactions between virai RNAs of subgroup I and Ⅱ of CMV and no nonspecific reactions against healthy control in tissue-prints of tobacco leaves.

• 九州大学の論文

著者

• Uchiba Takayuki

  Laboratory of Plant Pathology,Division of Plant Pathology and Pesticide science,Department of Applied Genetics and Pest Management,Graduate School of Bioresource and Bioenvironmental Sciences,Kyushu University

• Shigemune Naofumi

  Laboratory of Plant Pathology,Division of Plant Pathology and Pesticide science,Department of Applied Genetics and Pest Management,Graduate School of Bioresource and Bioenvironmental Sciences,Kyushu University

• Yoshitsugu Mami

  Program of Agronomy,Course of Agricultural Production,Engineering and Economics,School of Agriculture,Kyushu University

関連論文

• Subgroup-Specific Detection of Cucumber Mosaic Virus RNA in Tobacco Leaves by Tissue-Printing

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