Adjuvant Activities of Pyrene, Anthracene, Fluoranthene and Benzo (a) pyrene in Production of Anti-IgE Antibody to Japanese Cedar Pollen Allergen in Mice

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概要

• 論文の詳細を見る

• We have previously demonstrated that pyrene in diesel-exhaust particles (DEP) has an adjuvant activity on immunoglobulin E (IgE) antibody production in mice immunized with Japanese cedar pollen allergen (JCPA) or ovalbumin (OA) intraperitoneally. The present study is concerned with the adjuvant activity in IgE antibody production against JCPA of pyrene or DEP inoculated intranasally in mice. We show that anthracene, fluoranthene and benzo (a) pyrene in DEP have the ability to enhance anti-JCPA IgE antibody production in mice by intranasal immunization. Mice were grouped, immunized with 10μg of JCPA plus 400μg of pyrene, 10μg of JCPA plus 100μg of DEP, 10μg of JCPA plus 2mg of aluminum hydroxide and 10μg of JCPA alone intranasally 7 times at 2 week intervals. Mice were also grouped, and immunized with JCPA (10μg) plus 40μg of anthracene, JCPA (10μg) plus 400μg of fluoranthene, JCPA (10μg) plus 40μg of benzo(a)pyrene, and JCPA (10μg) plus 400μg of pyrene and JCPA (10μg) alone. We found that the IgE antibody responses to JCPA in mice immunized with JCPA plus pyrene, JCPA plus DEP or JCPA plus the three chemical organic compounds mentioned above were significantly enhanced compared with those immunized with JCPA alone. In addition, when the intraperitoneal macrophages obtained from the normal mice (unimmunized mice) were incubated with pyrene, anthracene, fluoranthene or benzo(a)pyrene in vitro, an enhanced chemiluminescence (Cl) response and interleukinlα (IL-1α) production of the macrophages was observed in each instance. These results suggest that in the production of IgE antibody to JCPA the adjuvancy of polycyclic aromatic hydrocarbons (PAHs) in DEP may be important in an attack of Japanese cedar pollinosis.

• 日本医科大学の論文
• 1997-03-25

著者

• Ohkuni H.

  Nippon Medical School

• Kanoh T.

  Miyazaki Medical College

• Suzuki T.

  The Tokyo Metropolitan Research Laboratory of Public Health

• Ishimori M.

  Tokyo Bunka Junior College

• Ikeda S.

  The Tokyo Metropolitan Research Laboratory of Public Health

• Ohasawa M.

  The Tokyo Metropolitan Research Laboratory of Public Health

• Tunetoshi Y.

  Miyazaki Medical College

関連論文

• Adjuvant Activities of Pyrene, Anthracene, Fluoranthene and Benzo (a) pyrene in Production of Anti-IgE Antibody to Japanese Cedar Pollen Allergen in Mice

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