Discrimination of three cyst-forming nematodes of the genus Globodera (Nematode : Heteroderidae) from Japan based on PCR-RFLP of ribosomal DNA
スポンサーリンク
概要
- 論文の詳細を見る
The ITS1-5.8S-ITS2 region of rDNA sequences from three Globodera species found in Japan, G. rostochiensis, G. tabacum and G. hypolysi, were sequenced and compared. Sequence lengths for G. rostochiensis, G. tabacum and G. hypolysi were 1,001, 1,003 and 1,016bp, respectively. Sequence divergence within the three Globodera species ranged from 3.8 to 14.7%. Eight restriction enzymes (AfaI, AluI, FspI, HhaI, HinfI, MvaI, PmaCI and SacII) were selected on the basis of sequence information. These three species were easily distinguished according to species-specific patterns by digesting the amplified products with AluI or PmaCI. The FspI-digested pattern distinguished G. rostochiensis from the other Globodera species.
- 日本応用動物昆虫学会の論文
- 2005-08-25
著者
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Kushida Atsuhiko
National Agricultural Res. Center For Hokkaido Region Hokkaido
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UEHARA Taketo
Laboratory of Plant Nematology, National Agricultural Research Center for Hokkaido Region
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KUSHIDA Atsuhiko
Laboratory of Plant Nematology, National Agricultural Research Center for Hokkaido Region
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ITOU Kenji
Laboratory of Plant Nematology, National Agricultural Research Center for Hokkaido Region
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NARABU Takashi
Laboratory of Plant Nematology, National Agricultural Research Center for Hokkaido Region
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MOMOTA Yoji
Laboratory of Plant Nematology, National Agricultural Research Center for Hokkaido Region
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Itou Kenji
National Agricultural Res. Center For Hokkaido Region National Agriculture And Food Res. Organizatio
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Uehara Taketo
Laboratory Of Plant Nematology National Agricultural Research Center For Hokkaido Region
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Momota Yoji
Laboratory Of Plant Nematology National Agricultural Research Center For Hokkaido Region:(present Ad
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Narabu Takashi
Laboratory Of Plant Nematology National Agricultural Research Center For Hokkaido Region
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Kushida Atsuhiko
Laboratory Of Plant Nematology National Agricultural Research Center For Hokkaido Region
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