魚粕の魚種鑑別法に関する研究(第二報)血清学的鑑別法

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About Antigen, 1. The method, by which some flesh meal of fish will be extracted with 5% Na Cl solution, is not so extractive upon the breaking action of the antigen, as by means of any other chemicals and makes better effects. 2. If the extracted solution, acts upon fish scrap, 30 minutes at 70℃ will be treated, is to attain the expected objects, though it cannot be avoid to cause somewhat decreasing of antigen action. 3. The antigenpreparation of cold-blooded animals can be more influenced than that of animals, bacteria by chemicals etc. About precipitation reaction, 1. Anti-serum for fish-scrap is hard to obtain, being taken very long time for it, but there is no use to take into consideration about any group reactions which may be accompanied by the anti-serum the reaction will be easy to prevent by adding a few glycerine or by inactivating. 2. Such a soluble protein, as it is extracted from some fish-scrap with 70℃ 30 minutes long and treated with alcohol, is to regard as the best one. About complement bindings reaction, 1. The reactions are almost equal to the preciptiation, but somewhat more accurate. 2. Such a reactinogen, as it is treated in the precipitation reaction with alcohol, is the best one. In the case of the above mentioned it must be avoided, to use a filter paper, because otherwise the principal constituents will be absorbed. About the identification of several fish-scrap, 1. The identification of several fish-scrap mixed in one kind of fish-scrap belongs to one of the most difficult works, but it is, ss far as the fish-scrap is mixed in some proportion with other ones, easy to detect the proportion of the mixture. 2. As the most of proteins of mackerel-cakes are decomposed as pepton and others, anti-bodies by that protein are hardly to get, but without the serum-reaction their mixture can be by the ninhydrine reaction easily found.
社団法人日本土壌肥料学会の論文
1940-10-25

魚粕の魚種鑑別法に関する研究(第二報)血清学的鑑別法

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