An Improved Method for Extraction and Measurement of the Inorganic Pyrophosphate in Leaves of Crassulacean Acid Metabolism (CAM) Plants
スポンサーリンク
概要
- 論文の詳細を見る
An improved method for extraction and measurement of inorganic pyrophosphate (PPi) in the leaves of crassulacean acid metabolism (CAM) plants, Ananas comosus (pineapple), Kalanchoe pinnata and K. dagremontiana is described. Leaf samples were extracted with 5% (w/v) trichloroacetic acid (TCA) dissolved in water. The extracts without neutralizing with KOH or K_2CO_3 and those without washing with diethyl ether to remove TCA were directly used to measure PPi with PPi-dependent phosphofructokinase (PPi-PFK, EC 2. 7. 1. 90) coupled with the oxidation of NADH. The method is sensitive and simple, the lower limit of the measurement was less than 1 nmol PPi mL^<-1> reaction mixture and the recoveries of the PPi added were more than 96%. Moreover, the initial PPi levels in TCA extracts could be maintained for at least one day. This method was advantageous for extracting and measuring a large number of samples at the same time. In addition, we found that neutralizing the extract before determination was disadvantageous to PPi recovery.
- 日本作物学会の論文
著者
-
Nose Akihiro
Faculty Of Agricultural Saga University
-
Chen Li-song
Faculty Of Agriculture Saga University
関連論文
- Effects of KCN, SHAM and Oxygen Concentrations on Respiratory Properties of Purified Mitochondria Isolated from Ananas comosus (Pineapple) and Kalanchoe daigremontiana(Crop Physiology and Ecology)
- Potential of the Common Ice Plant, Mesembryanthemum crystallinum as a New High-Functional Food as Evaluated by Polyol Accumulation(Crop Physiology & Ecology)
- Regulatory Properties of Phosphoenolpyruvate Carboxylase in Crassulacean Acid Metabolism Plants : Diurnal Changes in Phosphorylation State and Regulation of Gene Expression(Crop Physiology and Ecology)
- Location of New Gene for Late Heading in Rice, Oryza sativa L. Using Interchange Homozygotes
- Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species, Ananas comosus, Kalanchoe pinnata and K. daigremontiana
- Gas Exchange Analysis for Estimating Net CO_2 Fixation Capacity of Mangrove (Rhizophora stylosa) Forest in the Mouth of River Fukido, Ishigaki Island, Japan(Crop Physiology and Ecology)
- Invertase Activities in Sheath-blight Infected Rice Selection Lines and Fungus Culture Filtrates
- Linkage Analysis of a Lateness Gene ef4 in Rice, Oryza sativa L.
- Trisomic Analysis of a Lateness Gene ef2 in Rice, Oryza sativa L.
- Diurnal Changes in Photosynthesis in Sugarcane Leaves : II. Enzyme activities and metabolite levels relating to sucrose and starch metabolism
- Diurnal Changes in Photosynthesis in Sugarcane Leaves : I. Carbon dioxide exchange rate, photosynthetic enzyme activities and metabolite levels relating to the C_4 pathway and the Calvin cycle
- Thermal Characteristics of C_4 Photosynthetic Enzymes from Leaves of Three Sugarcane Species Differing in Cold Sensitivity
- Effects of High Night Temperature on Crassulacean Acid Metabolism (CAM) Photosynthesis of Kalanchoe pinnata and Ananas comosus(Crop Physiology and Ecology)
- An Improved Method for Extraction and Measurement of the Inorganic Pyrophosphate in Leaves of Crassulacean Acid Metabolism (CAM) Plants
- Potential of the Common Ice Plant, Mesembryanthemum crystallinum as a New High-Functional Food as Evaluated by Polyol Accumulation