Differential Responses of Azolla to Phosphorus Defficiency : II. Screening Method under Concentration Controlled Conditions
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概要
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The growth of Azolla in the field is often limited by the phosphorous supply. To develop a screening technique for the ability of Azolla to grow and fix N_2 more efficiently under P-deficient conditions, water culture experiments were conducted under concentration-controlled conditions. Azolla was grown in a 60-liter bucket with a P concentration ranging from 0.02 to 0.06 ppm. At 0.03 ppm, Azolla became P-deficient. Under P-deficient condition, Azolla pinnata strains #5 and #7001 produced a larger amount of biomass and showed a lower tissue P concentration than A. microphylla #4018 and A. mexicana #2026. The P concentration in the culture medium decreased from 0.03 to 0.02 ppm during the growth of Azolla. A device for the supply of acid or alkaline and P solutions was designed to maintain a constant pH and P concentration in the water culture medium. Even when the P concentration was kept at 0.03-0.035 ppm, Azolla suffered from P deficiency. Under this condition, the A. microphylla, A. mexicana, A. filiculoides, and A. nilotica strains produced a smaller amount of biomass and showed a higher tissue P content than the A. pinnata strains. Growth responses of Azolla species under P concentration-limited conditions were, thus, similar to those under quantity-limited conditions. Quantity-limited water culture is more convenient for screening a large number of Azolla strains for their ability to grow under P-deficient conditions.
- 社団法人日本土壌肥料学会の論文
著者
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Kushari D.p.
Department Of Botany Burdwan University
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Watanabe Iwao
The International Rice Research Institute
関連論文
- Differential Responses of Azolla to Phosphorus Defficiency : II. Screening Method under Concentration Controlled Conditions
- Differential Responses of Azolla to Phosphorus Deficiency : I. Screening Methods in Quantity Controlled-Condition
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