DETERMINATION OF CARBOHYDRATES IN HYDROLYSATES OF VOLCANIC ASH SOIL BY LIQUID CHROMATOGRAPHY WITH FLUORESCENCE SPECTROSCOPY

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概要

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• Volcanic ash soil sample with carbon content of 10.4%, C/N:16.3, obtained form grassland was analyzed for the determination of component sugars in two-step hydrolysates by using high performance liquid chromatography (HPLC). Experiments were repeated four times to evaluate the whole process of acid hydrolysis of soil and determination of carbohydrates by HPLC using an apparatus with an anion exchange column (Shimadzu ISA-07/S2504), chemical reaction box and fluorescence spectrophotometer which was originally developed by Kato and Kinoshita. Glucose, galactose, mannose, xylose were separated and arabinose, fructose, fructose were eluted in the same peak. Rhamnose and ribose were also eluted together in the same peak. This method is considered to be applicable for the determination of component sugars in soil hydrolysates.

• 社団法人日本土壌肥料学会の論文

著者

• Hamada Ryunosuke

  Tokyo University Of Agriculture And Technology

• ONO Akiko

  Tokyo University of Agriculture and Technology

関連論文

• DETERMINATION OF CARBOHYDRATES IN HYDROLYSATES OF VOLCANIC ASH SOIL BY LIQUID CHROMATOGRAPHY WITH FLUORESCENCE SPECTROSCOPY

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