STUDIES ON RICE SEED PROTEASE : VI. Metal Ion Activation of Rice Seed Peptidase

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概要

• 論文の詳細を見る

• Peptidase in rice seed was investigated with regard to metal ion activation. The embryo contained the highest activity of the peptidase which hydrolysed various synthetic peptides (L-Leu-Gly-Gly, L-Leu-Gly, Gly-Gly, Gly-L-Leu, Gly-L-Phe, GLy-L-Met, etc.). The enzyme showed an optimum pH of 7.6 for the hydrolysis of Gly-Gly. The hydrolysis of the peptides by the peptidase was activated by metal ion (Mn^<2+>, Co^<2+>) and inhibited by EDTA, except for the liberation of L-Leu from L-Leu-Gly-Gly where Mn^<2+> was inactive and Co^<2+> was inhibitory. The metal ion effect was shown to involve activation of the enzyme rather than that of the substrate. The enzyme as activated by the activators for thiol groups and was sensitive to inhibition by the thiol reagents. It appeared probable than Mn^<2+> or Co^<2+> was bound through a thiol group at the active site of the enzyme.

• 社団法人日本土壌肥料学会の論文

著者

• Kitagishi Kakuzo

  Faculty Of Agriculture Mie University

• HORIGUCHI Tsuyoshi

  (Present address)Faculty of Agriculture, Kagoshima University

• Horiguchi Tsuyoshi

  (present Address)faculty Of Agriculture Kagoshima University

関連論文

• Studies on rice seed protease V. Protease inhibitor in rice seed
• EFFECTS OF ZINC DEFICIENCY ON THE NITROGEN METABOLISM OF MERISTEMATIC TISSUES OF RICE PLANTS WITH REFERENCE TO PROTEIN SYNTHESIS
• EFFECT OF ZINC DEFICIENCY ON 80S RIBOSOME CONTENT OF MERISTEMATIC TISSUES OF RICE PLANT
• PROTEIN METABOLISM IN RICE SEEDLING : I. Effect of the Inhibitors of Protein Synthesis on the Degradation of Seed Protein during Germination
• STUDIES ON RICE SEED PROTEASE : VI. Metal Ion Activation of Rice Seed Peptidase

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