Characteristics of light-regulated iron transport system in barley chloroplasts
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概要
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Fe absorption was examined using chloroplasts, isolated from leaves of hydroponically cultured Fe-sufficient barley plants, to clarify the mechanism underlying the light-dependent Fe-influx into the chloroplasts. Inhibitors of Fe transport, ATP and proton flux were employed. Fe transport into the chloroplasts was partially inhibited by bathophenanthrolinedisulfonic acid (BPDS), which suggested the involvement of Fe^<3+>-reductase in the process of transmembrane Fe-influx. We also found that adenosine triphosphate (ATP) and proton (H^+) flux were not the cause of transmembrane Fe transport. The optimum pH for Fe transport across the chloroplast envelope was 8.0. However, a sharp increase in the Fe absorption was observed in pH range of 7.0 to 7.5. Fe transport into the chloroplasts was unaffected by the presence of Zn, Cu, Ni, Mn, Co or Cd in the medium at 1 x 10^<-5> or 1 x 10^<-4> M concentration.
- 一般社団法人日本土壌肥料学会の論文
著者
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TAKAHASHI M.
Laboratory of Athletic Medicine, Yokohama Municipal University
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Yoshimura E.
Dept. Appl. Biol. Chem. Univ. Tokyo
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Bughio N.
Dept. Appl. Biol. Chem., Univ. Tokyo
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Bughio N.
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo
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Yoshimura E.
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo
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Nishizawa N.K.
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo
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Mori S.
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo
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Nishizawa N.k.
Laboratory Of Plant Molecular Physiology Department Of Applied Biological Chemistry The University Of Tokyo
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