Methionine Sulfoximine Suppressed the Stimulation of Dark Carbon Fixation by Ammonium Nutrition in Wheat Roots
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概要
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To evaluate the role of NH_4^+ assimilates in dark carbon fixation in roots in providing carbon skeletons expended for NH_4^+ assimilation, the rate of dark carbon fixation in roots was measured using NaH^<14>CO_3. The ^<14>C-metabolites were analyzed in wheat (Triticum aestivum L.) plants grown in NH_4^+ media for various periods of time with or without methionine sulfoximine (MSX) treatment. The dark carbon fixation rate in the roots of wheat plants that had been grown with NH_4^+ for 1 d was approximately 6-fold higher than the rate in control roots. The stimulation of dark carbon fixation in NH_4^+-grown plants, however, was not observed in MSX-treated roots. In the roots of NH_4^+-grown plants, the concentration and ^<14>C-labeling of acidic metabolites such as citrate and malate considerably decreased whereas those of basic metabolites, especially asparagine, increased noticeably. With MSX treatment, the incorporation of ^<14>C into basic metabolites was negligible. In response to NH_4^+, phosphoenolpyruvate carboxylase (PEPC) activity increased, and PEPC proteins accumulated in wheat roots. Neither activity nor amounts of PEPC in roots increased in the presence of MSX. These findings suggest that primary assimilation of NH_4^+ in roots is essential for the stimulation of dark carbon fixation, which coincides with the increased activity of root PEPC, to sufficiently replenish carbon skeletons necessary for NH_4^+ assimilation.
- 社団法人日本土壌肥料学会の論文
著者
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Ikeda M
Laboratory Of Plant Nutrition Department Of Agricultural Chemistry Kyushu University
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Ikeda Motoki
Laboratory Of Plant Nutrition Division Of Soil Science And Plant Production Department Of Plant Reso
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Koga N
Kyushu Univ. Fukuoka Jpn
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Koga Nobuhisa
Laboratory of Plant Nutrition, Department of Agricultural Chemistry, Kyushu University
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Ikeda Motoki
Laboratory Of Plant Nutrition And Soil Fertility Faculty Of Agnculture Kyushu University
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