DNAのエンドジョイニングの分子機構:2種類の非相同的組換え : 第28回大会シンポジウム : II.新しい変異原検出法へのアプローチ

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Based on study of illegitimate recombination in Escherichia coli, we have classified illegitimate recombination into two types. One is a short homology-dependent illegitimate recombination (SHDIR) and the other is a short homology-independent illegitimate recombination (SHIIR) . SHIIR is mediated by DNA gyrase, and it usually takes place at a low frequency, but enhanced in the HU-defective mutant. Therefore this recombination is usually suppressed by HU protein. SHIIR may also be induced by a modification of DNA substrate, because the recombination induced by H_2O_2 seems to be of short homology-independent type. SHDIR takes place spontaneously or induced by irradiation with UV light or gamma ray and is widely detected in various genetic systems of E. coli. The 5'-3' exonuclease, exoVIII, enhances SHDIR by producing DNA ends with 3'-overhang, while the 3'-5' exonuclease, exoI, suppresses SHDIR by degrading the 3'-overhang from DNA ends. RecJ exonuclease also plays a role in UV-induced SHDIR. Models for SHIIR and SHDIR have been discussed.
日本環境変異原学会の論文
2000-07-31