Antimutagenicity testing of glucopyranosylvanillin and vanillin examined in a mouse peripheral blood micronucleus test system
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概要
- 論文の詳細を見る
Utilizing the fact that peak responses of micronuclei in peripheral blood appear approxi-mately 48 hr after a single chemical dosing and that blood samples can be collected consecutively from the same animal, an in vivo antimutagenicity testing system was designed on the basis of the micronucleus test with mutagen-sensitive MS/Ae mice. This system consisted of 1) sampling of peripheral blood before treatment for the baseline micronucleus frequency and administration of test chemicals soon after the sampling, 2) the second sampling of the blood 48 hr after the dosing to examine if test chemicals themselves induced micronuclei, 3) soon after the second sampling, administration of test chemicals followed by administration of a known micronucleus inducer cyclophos-phamide (CP, 20 mglkg) , and 4) the third sampling of the blood 48 hr after the combined dosing to examine if test chemicals suppressed micronucleus frequencies induced by CP. One group consisted of 6 MS/Ae mice, and 2000 reticulocytes per animal were examined for micronuclei. More than 500 erythrocytes per animal were scored for the ratio of reticulocytes to total erythrocytes. When 125, 250, and 500 mglkg of glucopyranosylvanil-lin (GV) , a synthesized compound with expectation to be antimutagenic, was given, 500 mg/kg stimulated the induction of micronuclei by CP, but 125 and 250 mglkg significantly repressed CP-induced micronucleus frequencies. When 61, 122, and 244 mg/kg (equiva-lents to three doses of GV) of vanillin, an in vitro antimutagen, were administered, 244 mg/kg also stimulated the micronucleus induction by CP and 122 mg/kg suppressed it. These results indicate that the present in vivo system is applicable to testing of in vivo antimutagens, that GV and vanillin are antimutagens, and that antimutagens might be mutation enhancers at higher doses.
- 日本環境変異原学会の論文
- 1999-10-29
著者
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MOCHIZUKI Masataka
Kyoritsu University of Pharmacy
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Sutou Shizuyo
National Institute Of Bioscience And Human-technology
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MITSUI Youji
National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology
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Mitsui Youji
National Institute Of Bioscience And Human-technology
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Mitsui Youji
National Institute Of Bioscience And Human Technology Agency Of Industrial Science And Technology
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Takada Masashi
Kyoritu Colledge Of Pharmacy
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Wong Liquing
Kyoritu Colledge of Pharmacy
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Mochizuki Masataka
Kyoritsu College Of Pharmacy
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Mochizuki Masataka
Kyoritu Colledge Of Pharmacy
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