遺伝子工学的手法を用いたアルキル化剤高感受性サルモネラ試験菌株の作製とその応用

概要

論文の詳細を見る
Of the DNA lesions generated by methylating agents, a minor alkylation product O^6-methylguanine is responsible for most of the mutations induced. To counteract such mutagenic effects, most organisms possess O^6-methylguanine DNA methyltransferases (MT) . This enzyme is ubiquitous in various organisms and has already been cloned from human, mouse, rat, and other species. It is reported that many cultured human cell lines are sensitive to alkylating agents compared to normal cells and in many cases such cell lines do not exhibit MT activity. Thus, the study of the role of MT may be important for cancer prevention. In this study, MT-deficient tester strains of Salmonella typhimurium were constructed using a newly developed preligation method. Since the new strains are highly sensitive only to alkylating agents, they will be useful to detect associated mutagenicity with high efficiency and to study the mechanism of mutagenesis induced by environmental alkylating agents. (This paper, in a session chaired by Yoshinari Ohnishi, is the lecture of The JEMS Achievement Award (1998) presented at the 27^<th> annual meeting of the Environmental Mutagen Society of Japan, held at the Mierparque in Osaka, Japan, November 24-26, 1998.)
日本環境変異原学会の論文
1999-07-12