Identification and characterization of genetic risk from germ cell mutagens (日本環境変異原学会第26回大会シンポジウム) -- (生殖細胞変異研究の新しい動向)

概要

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The germ cell tests detect chromosomal aberrations, gene mutations and aneuploidy induced in rodent germ cells by chemical or physical agents. The rodent germ cell tests are usually conducted by treating male animals. Recently, molecular cytogenetic techniques(fluorescent in situ hybridization, FISH)were developed to detect aneuploidy in sperm of experimental rodents and humans. A variety of assays are available which detect chromosomal aberrations in germ cells of treated animals, i.e.the germ cell cytogenetic tests, the dominant lethal assay and the reritable translocation test. Transgenic mice were recently used to detect gene mutations in germ cells. This assay promises to be faster and less animal consuming than the classical gene mutation tests in germ cells, the specific locus tests with various recessive and dominant marker genes. Recent data with the environmentally and occupationally important chemical 1, 3-butadiene were used to demonstrate the identification, characterization and quantification of potential genetic hazard for humans. Clastogenicity data in somatic and germinal cells of mice and in somatic cells of exposed workers were applied to the parallelogram approach for human risk assessment proposed by Sobels.
日本環境変異原学会の論文
1998-07-21