Characterization of a New DNA Polymerase Activity from Brassica oleracea, var. botrytis
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概要
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Background & Aims : A new DNA polymerase activity (temporally desinated-C) isolated from a rapidly growing apical tissue of cauliflower (Brassica oleracea, var. botrytis) inflorescence has been characterized and compared with cauliflower DNA polymerases, -A and -B,and with DNA polymeases α,βand γ from mouse myeloma. Methods : Enzyme activity was determined in vitro by measuring the incorporation of [^3H] dTMP into the DNA polymer under a variety of conditions (inhibitor : NEM, ddTTP, template-primer utilities and so on). Results : DNA polymerase-C from cauliflower had distinct elution pattern discriminated from these of polymerase-A and -B. Poly(rA)(dT)_<10> was utilized as template effectively for polymerase-C, but no significant utilization of polymerase-A was observed. Polymerase-C was sensitive to NEM and was resistant to ddTTP. Conclusions : These results strongly indicate that DNA polymerase-C from cauliflower is to be a new enzyme for DNA synthesis in cauliflower. (Kitakanto Med J 2003 ; 53 : 121〜127)
- 北関東医学会の論文
- 2003-05-01