Growth Characteristics of Agromonas oligotrophica on Ferulic Acid

概要

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Agromonas oligotrophica is an oligotrophic, dinitrogen-fixing soil bacterium which is found to grow on ferulic acid. This study aimed to characterize in more detail the growth of A. oligotrophica on ferulate and the degradation of ferulate. Ten- and 100-fold dilutions of conventional nutrient broth (NB/10 and NB/100,respectively) and yeast extract-supplemented mineral salts (MS) media were used for the batch growth experiments. In these media, the initial growth of A. oligotrophica was suppressed by ferulate in a dose-dependent manner. However, the cell biomass formed in the cultures was positively correlated with the amounts of ferulate used at ferulate concentrations between O and 5 mM, indicating that ferulate was the growth-limiting nutrient under the conditions. Thus, the growth yield on ferulate was calculated to be 59-67 g dry weight (mol ferulate)^<-1> in the NB/10 and the yeast extract-supplemented MS cultures, while in the NB/100 cultures a lower value [33 g dry weight (mol ferulate)^<-1> was obtained. In a resting cell experiment with ferulate, vanillate was identified by high performance liquid chromatography and the degradation pathway was expected to be analogous to the well-known pathway which includes vanillate and protocatechuate as intermediates.
日本微生物生態学会の論文
2000-09-30