Co-Culture of a Methanogenic Archaeon and a Methanotrophic Bacterium on Sterilized Soil in Large Test Tubes : Design for Soil-Mediated Co-Culture

概要

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in a design utilizing a sterilized soil-mediated co-culture of methanogenic archaeon and methanotrophic bacterium, the activities of both the methanogen and methanotroph were examined. It was confirmed that CH_4 was produced by the methanogen after methanotrophic inoculation and suggested that this CH_4 was oxidized by the methanotroph as the concentration of O_2 increased, although emission of CH_4 itself was indirect proof of CH_4-oxidizing activity. On the other hand, in the control treatment, methanogenic inoculation without methanotrophic inoculation at different levels of O_2, CH_4 was not emitted (there was no activity to produce CH_4). These results suggest a symbiotic relation in this co-culture system, because the methanogen provided CH_4 to the methanotroph, while inoculation of the methanotroph was necessary for subsequent production of CH_4 by the methanogen. The development of co-culture systems is essential for understanding the close relationship and interaction between methanogens and the methanotrophs in natural and agricultural environments.
日本微生物生態学会の論文