Two-Photon Imaging for Studying the Microbial Ecology of Biofilm Systems
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概要
- 論文の詳細を見る
Conventional confocal laser scanning microscopy uses continuous wave visible or UV lasers for one-photon excitation and imaging. These instruments are now enhanced with pulsed infrared lasers allowing two-photon excitation. With the appropriate technical setup two-photon excitation may be used for intensity imaging or for lifetime imaging. Both of these techniques have specific advantages if compared with one-photon excitation. Despite this fact, to date mainly one-photon excitation has been employed in microbial ecology. In this review the potential of two-photon intensity and lifetime imaging is discussed, using appropriate examples from cell biology as well as the first reports in microbiology.
- 日本微生物生態学会の論文
著者
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Neu Thomas
Department Of River Ecology Ufz Centre For Environmental Research Leipzig-halle
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WALCZYSKO PETR
Department of River Ecology, UFZ Centre for Environmental Research Leipzig-Halle
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LAWRENCE JOHN
NWRI National Water Research Institute
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Walczysko Petr
Department Of River Ecology Ufz Centre For Environmental Research Leipzig-halle