ミオシンのATP結合ポケットの構造変化と筋収縮

概要

論文の詳細を見る
Myosin is one of the key protein components of biological force generating systems. The globular head region of the myosin molecule, subfragment-1 (S-1), contains the site for ATP binding and hydrolysis and the site for actin binding. Although the open and closed conformational states of the ligand-binding pocket have been well established with various proteins other than myosin, such an operation of the ATP-binding pocket of S-1 is purely speculative at present. A hydrophobic fluorescent dye was found to bind to the ATP-binding pocket of S-1. Fluorescence study reveals that the ATP-binding pocket of S-1 is closed and / or tightened during ATP hydrolysis.
日本生物物理学会の論文
1995-05-25