In vitro 培養系にみる骨形成因子 (Bone Morphogenetic Protein) による筋肉, 滑膜由来細胞の軟骨への文化

概要

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Either muscle or synovium-derived mesenchymal-type cells differentiated into cartilage in response to a coat of partially purified rabbit bone morphogenetic protein (BMP) on a substratum of plastic. The crude BMP as a molecule, in slab electrophoresis, was observed to be separatod to 14K, 18K, 22K, and 35K zones, After dissolved BMP was coated on culturc dish, and then evaporated and dried, embryonic rat mesenchymal cells from muscle or adult rabbit syovial cell were cultured, Under lO%, fetal calf scrum, containing HamF12 medium, 1.8-5.OX105 cells/ml were cultivated on the several concentrated BMP coated culture dish. Observed by means of phase contrasL microscopy, light microscope histochemistry, and electron microscopy, the sequence of events on a BMP-coated plastic substratum is the same as observed in a substratum of bone matrix. The plastic eliminates the possibility of any endogenous allogeneic matrix-derived BMP contaminating the system. This observation suggests that immatured cells such as rat embryonic mesenchymal cell as well as matured synovial cells respond to BMP. Electron microscopic into observation suggested, that the endocytosis of BMP with responding cell were important the differentiation chondrocyte. The differentiation of cartilage from synoviocytes and subsynovial cells resembles the pathologic process occurring in human chondromatosis.
硬組織再生生物学会の論文
1996-07-10