骨形成タンパク質 (BMP) の遺伝子とその生物学的機能

概要

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Characterization of Xencpus BMP genes. We have previously demonstrated that activin, a member of the TGF-b family, has a potent mesoderm-inducing activity in Xenopus embryos. Activin can effrciently induce mesoderln formation that is associated with Eφ-activin gene activation when applied to the culture of isolated presumptive ectoderm (animal cap). In the course of screening for activin-related genes from Xenopus, we isolated a gene which predicts a protein which is extremely similar to human BMP-2 (bone morphogenetic protein-2) which had previously isolated for its ability to induce ectopic bone formation. Subsequently, we have cloned cDNAs for Xenopus homologue of BMP-2, -4 and -7 (Fig. 1A, 1B). Deduced protein structure of Xenopus BMP-2, -4 and 7 suggested that they all are synthesized as large precursor proteins of 398, 401, 428 amino acids, and processed to mature proteins of 114, 114, and 428 amino acids. Northern blot analysis revealed that these BMP genes are maternally encoded and differentially regulated after fertilization (Fig. 2).
硬組織再生生物学会の論文
1996-07-10