GBHA Staining Method: Its Application to Demonstration of Cytoplasmic Calcium Stores in Some Exocrine and Endocrine Glands

スポンサーリンク

概要

• 論文の詳細を見る

• The glyoxal bis(2-hydroxyanil)(GBHA) staining method for demonstration of cytoplasmic calcium has been tested on some exocrine and endocrine glands known to contain considerable amounts of calcium. Thick sections of rapidly frozen/freeze-substituted and p;astic embedded specimens were subjected for GBHA staining revealed variable degrees of Ca-GBHA reactions in the cytoplasm of glandular cells tested at a resolution to be able to depict those in the individual secretory granules, and further made it possible to distinguish between the reactive and non-reactive cells at the organ level. Moreover, GBHA staining revealed otherwise undetectable granular micro precipitates enriched with calcium and phosphorus in duct saliva within the submandibular gland and proved useful for evaluation of initial changes associated with ectopic mineralization known to occur in this gland. A precise method for GBHA staining and the diversity of this method for evaluation of calcium-related histo-pathological changes in various tissues were elaborated.

• 硬組織再生生物学会の論文
• 1993-10-31

著者

• 大島 勇人

  新潟大学大学院医歯学総合研究科摂食環境制御学講座顎顔面解剖学分野

• Sato Yasunori

  Department Of Human Pathology Kanazawa University Graduate School Of Medicine

• Maeda T

  Niigata Univ. Graduate School Of Medical And Dental Sci. Jpn

• Takano Y

  Tokyo Medical And Dental Univ. Tokyo Jpn

• Ohshima H

  Niigata Univ. Graduate School Of Medical And Dental Sci. Jpn

• Sato Yasunori

  Department Of Oral National Defense Medical College

• Takano Yoshiro

  Maxillofacial Surgery, National Defense Medical College

• Ohshima Hayato

  Maxillofacial Surgery, National Defense Medical College

• Maeda Takeyasu

  Maxillofacial Surgery, National Defense Medical College

• Sato Yasunori

  Department Of Cardiovascular Disease Tohoku Rosai Hospital

• Sato Yasunori

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University:Aging Regulation, Tokyo Metropolitan Institute of Gerontology

• Sato Yasunori

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University

関連論文

• Determination of Dehydroascorbic Acid in Mouse Tissues and Plasma by Using Tris(2-carboxyethyl)phosphine Hydrochloride as Reductant in Metaphosphoric Acid/Ethylenediaminetetraacetic Acid Solution
• Tumor necrosis factor-α induces the aberrant expression of mucus core protein-2 in non-neoplastic biliary epithelial cells via the upregulation of CDX2 in chronic cholangitis
• Pathology of peripheral intrahepatic cholangiocarcinoma with reference to tumorigenesis
• Involvement of Escherichia coli in pathogenesis of xanthogranulomatous cholecystitis with scavenger receptor class A and CXCL16-CXCR6 interaction
• Local balance of transforming growth factor-β1 secreted from cholangiocarcinoma cells and stromal-derived factor-1 secreted from stromal fibroblasts is a factor involved in invasion of cholangiocarcinoma
• Characterization of biliary intra-epithelial lymphocytes at different anatomical levels of intrahepatic bile ducts under normal and pathological conditions : Numbers of CD4^+CD28^- intra-epithelial lymphocytes are increased in primary biliary cirrhosis
• Histone deacetylase inhibitor (SAHA) and repression of EZH2 synergistically inhibit proliferation of gallbladder carcinoma
• A Treatment Using STI571 for Jejunal Gastrointestinal Stromal Tumor (GIST) Accompanied with Liver Metastasis and Peritoneal Dissemination
• Vitamin C Is Not Essential for Carnitine Biosynthesis in Vivo : Verification in Vitamin C-Depleted Senescence Marker Protein-30/Gluconolactonase Knockout Mice(Highlighted paper selected by Editor-in-chief,Biochemistry)
• Ciliated hepatic cyst without smooth muscle layer : A variant of ciliated hepatic foregut cyst?
• Autoimmune hepatitis associated with bile duct injury resembling chronic non-suppurative destructive cholangitis
• Fibulin-5 is involved in phlebosclerosis of major portal vein branches associated with elastic fiber deposition in idiopathic portal hypertension
• Small interfering RNA targeting epidermal growth factor receptor enhances chemosensitivity to cisplatin, 5-fluorouracil and docetaxel in head and neck squamous cell carcinoma
• Determination of triazolam by GC-MS in two autopsy cases : distribution in body fluids and organs
• GBHA Staining Method: Its Application to Demonstration of Cytoplasmic Calcium Stores in Some Exocrine and Endocrine Glands
• Possible Role of Heat Shock Protein (Hsp) 25 in the Enamel Organ during Amelogenesis in the Rat Molar
• Class II MHC Antigen-Expressing Cells in the Pulp Tissue of Human Deciduous Teeth Prior to Shedding
• Intraductal tubular neoplasm of the common bile duct
• Biliary deposition of Liesegang rings presenting as a polypoid mass in the liver : Previously unrecognized lesion
• Bile ductular cell reaction with senescent hepatocytes in chronic viral hepatitis is lost during hepatocarcinogenesis
• Intraductal papillary-mucinous neoplasm of the pancreas associated with polycystic liver and kidney disease
• Pathology and pathogenesis of portal venopathy in idiopathic portal hypertension : Hints from systemic sclerosis
• Submassive hepatocellular necrosis associated with infiltration by peripheral T-cell lymphoma of cytotoxic phenotype : Report of two cases
• Subpopulation of Class II MHC Antigen-expressing Cells in the Rat Incisor Pulp as Shown by Acid Phosphatase Histochemistry
• Transient Expression of Heat Shock Protein (Hsp) 25 in the Dental Pulp and Enamel Organ during Odontogenesis in the Rat Incisor
• Tumorigenesis and phenotypic characteristics of mucin-producing bile duct tumors : an immunohistochemical approach
• Responses of Odontoblasts to Cavity Preparation in Rat Molars as Demonstrated by Immunocytochemistry for Heat Shock Protein (Hsp) 25
• Alteration in the Expression of Heat Shock Protein (Hsp) 25-Immunoreactivity in the Dental Pulp of Rat Molars Following Tooth Replantation
• Characterization of hepatocellular adenoma based on the phenotypic classification : The Kanazawa experience
• Enhanced healing of mitomycin C-treated healing-impaired wounds in rats with hydrosheets composed of chitin/chitosan, fucoidan, and alginate as wound dressings

もっと見る 閉じる

スポンサーリンク