象牙質生検法の実際と生検試料の微細形態学的観察法

概要

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Previously, extracted teeth have been used for morphological reseach of tooth hard tissue, and it has been impossible to obtain spec mens from the teeth which were not subjected to extraction. Moreover, in extracted teeth, researchers have had difficulties to observe selected areas corresponding to the clinical symptoms. We have created a dentin biopsy method, and have observed the ultrastructures of dentin biopsy specimens obtained from both hypersensitive and insensitive areas of human hypersensitive teeth. In the present paper, we introduce the practice of the dentin biopsy method and decsribe how to handle dentin biopsy specimens for morphological observations. A cuboidal cylindrical diamond point was used with an air-turbin handpiece under water-cooling. The dentin biopsy specimens obtained from hypersensitive teeth were examined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and microradiography(MR). The dentin biopsy method can be applied to observe various kinds of tooth hard tissue diseases, and new attractive data from in vivo would be provided with use of the dentin biopsy method.
硬組織再生生物学会の論文
1993-03-27