Dental Pulp Cells with Multi-Potential for Differentiation to Odontoblast and Chondroblast

スポンサーリンク

概要

• 論文の詳細を見る

• The repair of dentin is thought to be maintained through differentiation of undefined precursor cells within the dental pulp to odontoblasts. To investigate the characteristics of these undefined precursor cells in dental pulp, mixed pulp cells and cloned cells were obtained from H-2K^b-ts A58 heterozygous transgenic mice. Differentiation of dental pulp precursor cells in vitro was assayed by RT-PCR analysis of gene expression of osteopontin, osteocalcin and dentin sialophosphoprotein (DSPP), and by von Kossa staining for mineralization. All assays were conducted on cultured mixed pulp cells and clonal cells PC1, PCS and PC 14. The results suggested that dental pulp cells could differentiate to odontoblasts in vitro. To test for chondrogenic potential, mixed pulp cells and clonal cells PC1, PCS and PC 14 were maintained in high density pellet cultures, and PC1 cells were stained with toluidine blue for glycosaminoglycans (GAG) content. To confirm the chondrocyte phenotype, the extracellular matrix of PC1 cells in pellet culture was stained with anti-type II collagen antibody. This study is the first to demonstrate that dental pulp stem cells present in the dental pulp have multi-potential to differentiate to both odontoblast and chondroblast.

• 硬組織再生生物学会の論文

著者

• Yamashita Kikuji

  Department Of Oral And Maxillofacial Anatomy Graduate School Of Health Biosciences University Of Tok

• Yamashita Kikuji

  Department Of Anatomy School Of Dentistry The University Of Tokushima

• Yamashita Kikuji

  First Department Of Oral Anatomy School Of Dentistry University Of Tokushima

• KITAMURA SEIICHIRO

  Department of Oral Anatomy, Tokushima University School of Dentistry

• Caplan Arnold

  Skeletal Research Center Department Of Biology Case Western Reserve University

• MORIMOTO Hiroyuki

  Department of Anatomy, School of Dentistry, The University of Tokushima

• Morimoto Hiroyuki

  Department Of Oral And Maxillofacial Anatomy Medical Science For Oral And Maxillofacial Regeneration

• Morimoto Hiroyuki

  Department Of Anatomy School Of Dentistry The University Of Tokushima

• Kitamura Seiichiro

  Department Of Oral And Maxillofacial Anatomy Graduate School Of Health Biosciences University Of Tok

• Kitamura Seiichiro

  Department Of Anatomy School Of Dentistry The University Of Tokushima

• DENNIS James

  Skeletal Research Center, Department of Biology, Case Western Reserve University

• LENNON Donald

  Skeletal Research Center, Department of Biology, Case Western Reserve University

• Dennis James

  Skeletal Research Center Department Of Biology Case Western Reserve University

• Yamashita Kikuji

  Department Of Oral And Maxillofacial Anatomy Medical Science For Oral And Maxillofacial Regeneration

• Lennon Donald

  Skeletal Research Center Department Of Biology Case Western Reserve University

• Kitamura Seiichiro

  Department Of Oral And Maxillofacial Anatomy Graduate School Of Health Biosciences University Of Tok

• Kitamura Seiichiro

  First Department Of Oral Anatomy School Of Dentistry University Of Tokushima

• Morimoto Hiroyuki

  Deparment Of Oral Anatomy Kyushu Dental College

• Yamashita Kikuji

  Department Of Oral And Maxillofacial Anatomy Graduate School Of Health Biosciences University Of Tok

関連論文

• Effects of Carbonic Anhydrase Inhibitor Acetazolamide (AZ) on Osteoclasts and Bone Structure
• Blood supply to the duodenal papilla and the communicating artery between the anterior and posterior pancreaticoduodenal arterial arcades
• Effects of TGF-β1 on Extracellular Matrix Formation in Rat Clonal Dental Pulp Cells
• Okadaic Acid Induces Apoptosis through Double-Stranded RNA-Dependent Protein Kinase/Eukaryotic Initiation Factor-2α Pathway in Human Osteoblastic MG63 Cells
• The Goitrogenic Action of Manganese (Regular Presentations) (Proceedings of the 11 th Symposium on Environmental Pollutants and Toxicology)
• Morphological Features of the Osteopetrotic Op/Op Mouse Skeleton
• Microarray Analysis of Gene Expression in MC3T3-E1 after Specific Far-infrared Radiation(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
• Induction of regenerative hard tissues(13^ Annual Meeting of the Society for Hard Tissue Regenerative Biology)
• Extracellular Matrix Formed by MC3T3-E1 Osteoblast-Like Cells Cultured on Titanium : II. Collagen Fiber Formation
• Extracellular Matrix Formed by MC3T3-E1 Osteoblast-Like Cells Cultured on Titanium : I. Anchor Structure
• Dental Pulp Cells with Multi-Potential for Differentiation to Odontoblast and Chondroblast
• Histological Study on Endochondral Ossification in the Osteopetrotic Op/op Mouse
• Effects of far-infrared radiation (FIR) on osteoblast-like cells (MC3T3-E1) and bone formation(Oral Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
• Forming mechanism of extracellular matrix containing periodontal ligament-like collagen fiber and its bone inducing activity(15thAnnual Meeting of the Society for Hard Tissue Regenerative Biology,Session 2)
• Effects of carbonic anhydrase inhibitor acetazolamide (AZ) on osteoclasts and bone texture(Poster Session,The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology in Matsumoto Dental University)
• Type 1 Protein Phosphatase Controls Biological Processes in Cultured Human Osteoblastic Cells
• Renal cell carcinoma with hemorrhagic cyst formation in a 4-year-old boy
• THE USE OF MESENCHYMAL STEM CELLS TO REGENERATE SKELETAL TISSUES
• Mesenchymal stem cells: Progenitors, progeny, and pathways
• Inhibitors of protain synthesis and RNA synthesis protect against okadaiacid-induced apoptosis in humansarcoma cell line MG63 cells but not in Soos-2 cells
• Transient immunoglobulin-complexed aspartate aminotransferase in infancy caused by maternal immunoglobulin G
• Interaction of Protein Phosphatase 1δ with Nucleophosmin in Human Osteoblastic Cells

もっと見る 閉じる

スポンサーリンク