人工飼料に用いるダイズ粉末標品の違いがBacillus thuringiensis製剤の殺虫活性に与える影響

概要

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The insecticidal activity of Bacillus thuringiensis (BT) formulation for silkworms (Bombyx mori) is affected by the defatted soybean meal manufacturing and refinement. The LC_<50> of silkworms reared on artificial diets with SP2 and SP600 soybean meals (3.02 and 3.38 μg/g diet, respectively) was significantly higher than the LC_<50> of silkworms reared on artificial diets with STN meal (2.41 μg/g diet). STN soybean meal is an ordinary meal (Katakura Kogyo Co., Tokyo) used by ACIS(Agricultural Chemicals Inspection Station). SP2 and SP600 are products of another manufacturer (Nissin Cosmo Food Co., Tokyo), but the refinement processes are different. SP600 contains more protein (68%) and less breakdown products (NSI>30%) than SP2 (53% protein, NSI<30%). Western blot analysis using a monoclonal antibody against CrylAa (a BT δ-toxin) showed CrylAa bindable proteins in the soybean meals. The band-intensity of each meal was different (STN<SP2<SP600). It is suggested that these proteins in the soybean meals bind to CrylAa competitively and inhibit the activity of the BT formulation used in these experiments.
2000-05-25