In Vitro Rearing of an Endoparasitic Fly, Exorista sorbillans (Diptera: Tachinidae)
スポンサーリンク
概要
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In order to establish an in vitro rearing method for an endoparasitic fly, Exorista sorbillans, various liquid media originally formulated for parasitoid rearing or for insect cell cultures were tested. About 50% of parasitoid larvae molted to the 2nd instar in the basic medium, an insect cell culture medium, MGM450, when it was fortified with 10% Bombyx mori hemolymph and 10% fetal bovine serum, whereas fewer or no larvae did so in the parasitoid rearing media formulated by other workers. Qualitative and quantitative differences were observed in hemolymph amino acids between parasitized and unparasitized 7-d-old 5th instar larvae of the host, B. mori. Heat-treated hemolymph of the host alone enabled the parasitoid larvae to develop to the pupal stage. Among the hemolymph samples taken from host larvae during the first 4 d of the 5th instar, the sample obtained from 4-d-old 5th instar larvae proved to be the best medium, and it sustained the development of larvae to the pupal stage. When such hemolymph was mixed with a modified melon fly diet or with wheat bran, some individuals of E. sorbillans completed development to the adult stage.
- 1995-05-25
著者
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Mitsuhashi Jun
Laboratory Of Applied Entomology Faculty Of Agriculture Tokyo University Of Agriculture And Technolo
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Mitsuhashi Jun
Laboratory Of Applied Entomology Faculty Of Agriculture Tokyo University Of Agriculture And Technolo
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Watanabe Masahiko
Laboratory Of Applied Entomology Faculty Of Agriculture Tokyo University Of Agriculture And Technolo
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Watanabe Masahiko
Laboratory for Medical Engineering, Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
関連論文
- Developmental Responses of Galleria mellonella (Lepidoptera, Pyralidae) Larvae to Parasitism by a Braconid Parasitoid, Apanteles galleriae (Hymenoptera, Braconidae)
- Cuticular Proteins Specific to the Last Larval Stadium in the Sweet Potato Hornworm, Agrius convolvuli (Lepidoptera: Sphingidae)
- In Vitro Rearing of an Endoparasitic Fly, Exorista sorbillans (Diptera: Tachinidae)
- Partial Rearing of a Phytophagous Lady Beetle, Epilachna vigintioctopunctata (Coleoptera: Coccinelidae)
- Differences in Amino Acid Metabolism among Cell Lines Derived from Cabbage Armyworm, Mamestra brassicae : Lepidoptera : Noctuidae