A Granulosis Virus of the Tea Tortrix, Homona magnanima DIAKNOFF (Lepidoptera : Tortricidae) : Its Pathogenicity and Mass-Production Method

概要

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Pathogenicity and mass-production method of a granulosis virus of the tea tortrix, Homona magnanima DIAKNOFF were studied, Capsules were oval in shape and had an average size of about 385×220 nm. The virus rods within the capsules were about 200×55 nm in size. None of the trotricid larvae of the 6 species used in the cross-transmission study, except for the tea tortrix larvae, were infected with the virus. The original inoculum showed a high infectivity to the newly hatched larvae of the tea tortrix. The susceptibility of the larva to the virus decreased with the larval development. Mass-production of the virus was successful using a modified method for producing the granulosis virus of Adoxophyes orana fasciata. With scanning electron microscope, the capsules were observed to be attached on the surfaces of the egg-shells which were treated with so-called egg-mass dipping method. The rates of infection of the larvae hatched from the treated egg-masses depended on the concentration of inoculum. The moderate larval density for mass-production in the rearing container (28×20×6 cm) is considered to be about 100 mature larvae.
日本応用動物昆虫学会の論文
1980-11-25